The late 18th century to early 19th century elm collection box was acquired by a former cleaner of St Marks Church on High Street before it was demolished in 1971.
The box, which is estimated to fetch between £50 and £80, will be auctioned at the next sale at Golding Young & Thos. Mawer on Wednesday, February 16.
Auctioneer John Leatt said: "This box has two locks which is typical of collection boxes of the period. Usually the vicar and the church warden had a key each and both would have to be present before the box could be opened.
"The box came from a local church and so we expect it to appeal to local historians and collectors of Lincoln-area memorabilia."
In the sale, over 500 lots in the antique section will be sold over the Internet through a live bidding system.
A two-handled silver cup discovered in a local attic, which features the inscription 'Presented by Captain H. Bradwell to the 1st V.B. Lincoln Regiment, 1904', is expected to fetch £200 to £300 in the sale and attract the interest of local collectors of military history.
A rare poster from the early days of cinema, showing Lincolnshire volunteers returning from the campaign in South Africa (1899-1902), meeting the Mayor and marching through the city, is expected to fetch between £40 and £60. The poster states that 'Mr A. H. Vidler of Lincoln begs to announce his popular musical and dioramic entertainment'.
The books section includes a good selection of titles about Lincolnshire and Lincoln and the first part of an extensive local collection of topographical books on Britain.
Among the furniture is a Queen Anne period walnut
chest on a stand which is estimated to fetch between £1,200 to £1,500.
The highlight of a section of around 100 lots of good quality silver and jewellery is a two-handled rose bowl bearing the crest of the Manvers family of Holme Pierrepont Hall, Nottingham and Thoresby Hall in Nottinghamshire, which is estimated to make between £700 and £900.
The sale follows the hugely successful January auction which saw both salerooms packed with bidders, high numbers of internet buyers and numerous telephone bids for the top lots.
Items consigned from a large property in Nottingham achieved the highest prices in the sale. In particular, a collection of Royal Worcester animal figures designed by Doris Lindner sold for significantly more than the estimate each time with fierce competition from Internet buyers from the UK and American and bidders in the room. The collection made a total hammer price of £4,200.
A Royal Worcester fruit decorated part coffee service sold for £2,300 and was bought by one of a number of telephone bidders competing with others on the Internet.
Silver continued to make strong prices with a Winston Churchill commemorative cigar box, designed by the famous 20th century silversmith Stuart Devlin, and numerous other lots of silver making considerably more than their scrap values.
In the furniture section, a George II giltwood mirror sold for £2,400, a nest of three early-19th century rosewood tables made £900 and a George II small side table made £1,100.
Meanwhile, among the items entered into the sale by other vendors, an unusual collection of Polish military cap badges made £1,100, reinforcing the fact that the market is strong for militaria and collectables, and a four-tier mahogany Globe Wernicke bookcase fetched £580 and was bought by a continental buyer on a commission bid.